Molecular Investigation of Hemotropic Mycoplasmas in Human Beings, Dogs and Horses in a Rural Settlement in Southern Brazil

نویسندگان

  • Rafael Felipe da Costa VIEIRA
  • Odilon VIDOTTO
  • Thállitha Samih Wischral Jayme VIEIRA
  • Ana Márcia Sá GUIMARAES
  • Andrea Pires dos SANTOS
  • Naíla Cannes NASCIMENTO
  • Nelson Jesse Rodrigues dos SANTOS
  • Thiago Fernandes MARTINS
  • Marcelo Bahia LABRUNA
  • Mary MARCONDES
  • Alexander Welker BIONDO
  • Joanne Belle MESSICK
چکیده

The aims of this study were to determine the prevalence of hemoplasmas in a rural Brazilian settlement's population of human beings, their dogs and horses, highly exposed to tick bites; to identify the tick species parasitizing dogs and horses, and analyze factors associated with their infection. Blood samples from 132 dogs, 16 horses and 100 humans were screened using a pan-hemoplasma SYBR green real-time PCR assay followed by a species-specific TaqMan real-time PCR. A total of 59/132 (44.7%) dog samples were positive for hemoplasmas (21 Mycoplasma haemocanis alone, 12 ' Candidatus Mycoplasma haematoparvum' alone and 21 both). Only 1/100 (1.0%) human sample was positive by qPCR SYBR green, with no successful amplification of 16S rRNA or 23 rRNA genes despite multiple attempts. All horse samples were negative. Dogs >1 year of age were more likely to be positive for hemoplasmas ( p= 0.0014). In conclusion, although canine hemoplasma infection was highly prevalent, cross-species hemoplasma transmission was not observed, and therefore may not frequently occur despite overexposure of agents and vectors.

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عنوان ژورنال:

دوره 57  شماره 

صفحات  -

تاریخ انتشار 2015